Yeast Sequencing Report

The 5-aminoimidazole ribonucleotide-carboxylase structural gene of the methylotrophic yeast Pichia methanolica: Cloning, sequencing and homology analysis

  1. Tran Tuan Hiep,
  2. Vladimir N. Kulikov,
  3. Vladimir N. Noskov,
  4. Genadij I. Sizonenko,
  5. Yuri O. Chernoff,
  6. Yuri I. Pavlov*

Article first published online: 29 JAN 2004

DOI: 10.1002/yea.320091112

Issue

Yeast

Yeast

Volume 9, Issue 11, pages 1251–1258, November 1993

Additional Information

How to Cite

Hiep, T. T., Kulikov, V. N., Noskov, V. N., Sizonenko, G. I., Chernoff, Y. O. and Pavlov, Y. I. (1993), The 5-aminoimidazole ribonucleotide-carboxylase structural gene of the methylotrophic yeast Pichia methanolica: Cloning, sequencing and homology analysis. Yeast, 9: 1251–1258. doi: 10.1002/yea.320091112

Author Information

  1. Department of Genetics, Sankt-Petersburg University, Universitetskaya emb. 7/9, Sankt-Petersburg, 199034, Russia

*Department of Genetics, Sankt-Petersburg University, Universitetskaya emb. 7/9, Sankt-Petersburg, 199034, Russia

Publication History

  1. Issue published online: 29 JAN 2004
  2. Article first published online: 29 JAN 2004
  3. Manuscript Accepted: 10 JUN 1993
  4. Manuscript Received: 9 FEB 1993

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Keywords:

  • Methylotrophic yeasts;
  • phosphoribosyl-5-aminoimidazole-carboxylase;
  • gene homology analysis

Abstract

The ADE1 gene of the yeast Pichia methanolica encodes phosphoribosyl-5-aminoimidazole-carboxylase (AIRC, EC 4.1.1.21), which is involved in purine biosynthesis. The gene was cloned by complementation of an ade2 mutation in Saccharomyces cerevisiae and a 3077 nucleotide DNA fragment was sequenced. The sequence possessed a single open reading frame, corresponding to a 543 amino acid sequence. The sequence of this putative protein has been compared to the proteins of homologous genes from S. cerevisiae, Schizosaccharomyces pombe, Escherichia coli, chicken and man. The analysis revealed remarkable homology between yeast AIRCs, while for other proteins homology was limited to defined regions.

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