Isolation and characterization of a novel yeast gene, ATH1, that is required for vacuolar acid trehalase activity
Article first published online: 29 JAN 2004
Copyright © 1995 John Wiley & Sons Ltd.
Volume 11, Issue 11, pages 1015–1025, 15 September 1995
How to Cite
Destruelle, M., Holzer, H. and Klionsky, D. J. (1995), Isolation and characterization of a novel yeast gene, ATH1, that is required for vacuolar acid trehalase activity. Yeast, 11: 1015–1025. doi: 10.1002/yea.320111103
- Issue published online: 29 JAN 2004
- Article first published online: 29 JAN 2004
- Manuscript Accepted: 2 APR 1995
- Manuscript Received: 6 JAN 1995
- stationary phase;
- stress response;
We have isolated a plasmid containing a gene, ATH1, that results in eight- to ten-fold higher acid trehalase activity in yeast cells when present in high copy. The screening procedure was based on overproduction-induced mislocalization of acid trehalase activity; overproduction of vacuolar enzymes that transit through the secretory pathway leads to secretion to the cell surface. A DNA fragment that confers cell surface expression of acid trehalase activity was cloned and sequenced. The deduced amino acid sequence displayed no homology to known proteins, indicating that we have identified a novel gene. A deletion in the genomic copy of the ATH1 gene eliminates vacuolar acid trehalase activity. These results suggest that ATH1 may be the structural gene encoding vacuolar acid trehalase or that the gene product may be an essential regulatory component involved in control of trehalase activity. The sequence has been deposited in the GenBank data library under Accession Number X84156 S. cerevisiae ATH1 gene.