Yeast Sequencing Report

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Cloning and characterization of the Hansenula polymorpha homologue of the Saccharomyces cerevisiae MNN9 gene

  1. So-Young Kim1,2,
  2. Jung-Hoon Sohn1,
  3. Hyun Ah Kang1,
  4. Seung-Ku Yoo2,
  5. Yu-Ryang Pyun2,
  6. Eui-Sung Choi1,*

Article first published online: 14 FEB 2001

DOI: 10.1002/yea.699

Issue

Yeast

Yeast

Volume 18, Issue 5, pages 455–461, 30 March 2001

Additional Information

How to Cite

Kim, S.-Y., Sohn, J.-H., Ah Kang, H., Yoo, S.-K., Pyun, Y.-R. and Choi, E.-S. (2001), Cloning and characterization of the Hansenula polymorpha homologue of the Saccharomyces cerevisiae MNN9 gene. Yeast, 18: 455–461. doi: 10.1002/yea.699

Author Information

  1. 1

    Division of Life Sciences, Korea Research Institute of Bioscience and Biotechnology, Taejon 305–333, Korea

  2. 2

    Department of Food and Biotechnology, Yonsei University, Seoul 120–749, Korea

*Division of Life Sciences, Korea Research Institute of Bioscience and Biotechnology, 52 Oun-dong, Yusong-gu, Taejon 305–333, South Korea.

Publication History

  1. Issue published online: 14 FEB 2001
  2. Article first published online: 14 FEB 2001
  3. Manuscript Accepted: 6 NOV 2000
  4. Manuscript Received: 28 AUG 2000

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Keywords:

  • yeast;
  • Hansenula polymorpha;
  • MNN9;
  • glycosylation

Abstract

A gene homologous to Saccharomyces cerevisiae MNN9 has been cloned and characterized in the methylotrophic yeast Hansenula polymorpha. This gene was cloned from a H. polymorpha genomic DNA library using the S. cerevisiae MNN9 gene as a probe. The H. polymorpha MNN9 homologue (HpMNN9) contained a 1062 bp open reading frame encoding a predicted protein of 354 amino acids. The deduced amino acid sequence showed 58% and 51% identity, respectively, with the S. cerevisiae and Candida albicans Mnn9 proteins. Disruption of HpMNN9 leads to phenotypic effects suggestive of cell wall defects, including detergent sensitivity and hygromycin B sensitivity. The hygromycin B sensitivity of S. cerevisiae mnn9 null mutant was complemented in the presence of the HpMNN9 gene. The DNA sequence of the H. polymorpha homologue has been submitted to GenBank with the Accession No. AF264786. Copyright © 2001 John Wiley & Sons, Ltd.

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