Yeast Sequencing Report

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Cloning of glycerol-3-phosphate dehydrogenase genes (ZrGPD1 and ZrGPD2) and glycerol dehydrogenase genes (ZrGCY1 and ZrGCY2) from the salt-tolerant yeast Zygosaccharomyces rouxii

  1. Tomoko Iwaki,
  2. Sachko Kurono,
  3. Yuki Yokose,
  4. Kenji Kubota,
  5. Youichi Tamai,
  6. Yasuo Watanabe*

Article first published online: 30 MAR 2001

DOI: 10.1002/yea.722

Issue

Yeast

Yeast

Volume 18, Issue 8, pages 737–744, 15 June 2001

Additional Information

How to Cite

Iwaki, T., Kurono, S., Yokose, Y., Kubota, K., Tamai, Y. and Watanabe, Y. (2001), Cloning of glycerol-3-phosphate dehydrogenase genes (ZrGPD1 and ZrGPD2) and glycerol dehydrogenase genes (ZrGCY1 and ZrGCY2) from the salt-tolerant yeast Zygosaccharomyces rouxii. Yeast, 18: 737–744. doi: 10.1002/yea.722

Author Information

  1. Department of Biological Resources, Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan

*Laboratory of Biochemistry, Department of Biological Resources, Faculty of Agriculture, Ehime University, Matsuyama, Tarumi 3-5-7, Ehime 790-8566, Japan.

Publication History

  1. Issue published online: 30 MAR 2001
  2. Article first published online: 30 MAR 2001
  3. Manuscript Accepted: 24 DEC 2000
  4. Manuscript Received: 9 OCT 2000

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Keywords:

  • salt-tolerant yeast;
  • Zygosaccharomyces rouxii;
  • glycerol-3-phosphate dehydrogenase;
  • glycerol dehydrogenase

Abstract

The ZrGPD1 and ZrGPD2 genes encoding putative glycerol-3-phosphate dehydrogenases were isolated from the salt-tolerant yeast, Zygosaccharomyces rouxii. Both genes are homologous to GPD1 of Saccharomyces cerevisiae and are constitutively expressed in Z. rouxii cells. Putative glycerol dehydrogenase genes, ZrGCY1 and ZrGCY2, which are highly homologous to GCY1 of S. cerevisiae, were also isolated. Since the level of transcripts of ZrGCY1 and ZrGCY2 increased in Z. rouxii cells subjected to salt stress, it is suggested that the pathway of the signal transduction of salt stress controls the expression of these genes. The Accession Nos of these sequences in GenBank are as follows: ZrGPD1, AB047394; ZrGPD2, AB047395; ZrGCY1, AB047396; ZrGCY2, AB047397. Copyright © 2001 John Wiley & Sons, Ltd.

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