Yeast Sequencing Report

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The sequence of a 15 769 bp segment of Pichia anomala identifies the SEC61 and FBP1 genes and five new open reading frames

  1. Teresa Ruíz1,
  2. Manuel Sánchez2,
  3. José M. De la Rosa1,
  4. Luis Rodríguez1,*,
  5. Angel Domínguez2

Article first published online: 26 JUL 2001

DOI: 10.1002/yea.766

Issue

Yeast

Yeast

Volume 18, Issue 13, pages 1187–1195, 30 September 2001

Additional Information

How to Cite

Ruíz, T., Sánchez, M., De la Rosa, J. M., Rodríguez, L. and Domínguez, A. (2001), The sequence of a 15 769 bp segment of Pichia anomala identifies the SEC61 and FBP1 genes and five new open reading frames. Yeast, 18: 1187–1195. doi: 10.1002/yea.766

Author Information

  1. 1

    Departamento de Microbiología y Biología Celular, Facultad de Farmacia, Universidad de La Laguna, 38071 La Laguna, Tenerife, Spain

  2. 2

    Departamento de Microbiología y Genética, Instituto de Microbiología Bioquímica, CSIC-Universidad de Salamanca, 37071 Salamanca, Spain

*Departamento de Microbiología y Biología Celular, Facultad de Farmacia, Universidad de La Laguna, 38071 La Laguna, Tenerife, Spain.

Publication History

  1. Issue published online: 26 JUL 2001
  2. Article first published online: 26 JUL 2001
  3. Manuscript Accepted: 3 MAY 2001
  4. Manuscript Received: 16 MAR 2001

Funded by

  • Dirección General de Universidades e Investigación, Gobierno de Canarias. Grant Number: PI1999/054
  • Ministerio de Educación y Cultura, Spain

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Keywords:

  • Pichia anomala;
  • Saccharomyces cerevisiae;
  • Candida albicans;
  • gene order;
  • genome organization;
  • intermingled triples

Abstract

We have determined the sequence of a 15 769 bp DNA segment of Pichia anomala. The sequence contains seven complete open reading frames (ORFs) longer than 100 amino acids and a putative tRNA gene. Two of the ORFs code for the well-characterized genes SEC61 (which codes for the core subunit of the ER translocation complex) and FBP1 (encoding fructose-1,6-bisphosphatase). A gene coding for a protein similar to S. cerevisiae YDL054c was found between the two genes. These three genes show a different organization (intermingled triples) in three yeast species: Saccharomyces cerevisiae, Candida albicans and P. anomala. Two out of the four remaining ORFs show weak homology with different proteins from other species and the other two show non-significant similarity with previously sequenced genes. The nucleotide sequence has been submitted to the EMBL database under Accession No. AJ306295. Copyright © 2001 John Wiley & Sons, Ltd.

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