Functional Analysis Report

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Functional analysis of yeast gene families involved in metabolism of vitamins B1 and B6

  1. Susana Rodríguez-Navarro1,†,‖,
  2. Bertrand Llorente2,‡,‖,
  3. María Teresa Rodríguez-Manzaneque3,
  4. Anna Ramne4,§,
  5. Genoveva Uber1,
  6. Denis Marchesan4,¶,
  7. Bernard Dujon2,
  8. Enrique Herrero3,
  9. Per Sunnerhagen4,
  10. José E. Pérez-Ortín1,*

Article first published online: 23 SEP 2002

DOI: 10.1002/yea.916

Issue

Yeast

Yeast

Volume 19, Issue 14, pages 1261–1276, October 2002

Additional Information

How to Cite

Rodríguez-Navarro, S., Llorente, B., Rodríguez-Manzaneque, M. T., Ramne, A., Uber, G., Marchesan, D., Dujon, B., Herrero, E., Sunnerhagen, P. and Pérez-Ortín, J. E. (2002), Functional analysis of yeast gene families involved in metabolism of vitamins B1 and B6. Yeast, 19: 1261–1276. doi: 10.1002/yea.916

Author Information

  1. 1

    Departamento de Bioquímica y Biología Molecular, Universitat de València, C/Dr Moliner 50, E-46100, Burjassot, Spain

  2. 2

    Unité de Génétique Moléculaire des Levures (URA 2171 du CNRS, UFR 927 Université Pierre et Marie Curie), Institut Pasteur, Paris, France

  3. 3

    Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina, Universitat de Lleida, Rovira Roure 44, E-25198 Lleida, Spain

  4. 4

    Department of Cell and Molecular Biology, Lundberg Laboratory, Göteborg University, PO Box 462, S-40530 Göteborg, Sweden

  1. †

    Biochemie-Zentrum-Heidelberg (BZH), Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.

  2. §

    Department of Molecular and Cellular Engineering, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6160, USA.

  3. ‡

    Department of Microbiology and Institute of Cancer Research, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA.

  4. Department of Medical Biochemistry, Göteborg University, PO Box 440, S-40530 Göteborg, Sweden.

  1. ‖

    These authors contributed equally to this work

*Departamento de Bioquímica y Biología Molecular, Universitat de Valéncia, C/Dr Moliner 50, E-46100, Burjassot, Spain.

Publication History

  1. Issue published online: 23 SEP 2002
  2. Article first published online: 23 SEP 2002
  3. Manuscript Accepted: 30 JUL 2002
  4. Manuscript Received: 7 MAY 2002

Funded by

  • Eu. Grant Number: B104-CT97-2294
  • Swedish Natural Science Research Council. Grant Number: 2000-5471
  • CICYT, Spain. Grant Number: B1098-1316-CE
  • Generalitat de Catalunya, Spain. Grant Number: 1999SGR 00170

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Keywords:

  • Saccharomyces cerevisiae;
  • thiamine;
  • pyridoxal;
  • functional analysis;
  • SNZ;
  • SNO

Abstract

In order to clarify their physiological functions, we have undertaken a characterization of the three-membered gene families SNZ1–3 and SNO1–3. In media lacking vitamin B6, SNZ1 and SNO1 were both required for growth in certain conditions, but neither SNZ2, SNZ3, SNO2 nor SNO3 were required. Copies 2 and 3 of the gene products have, in spite of their extremely close sequence similarity, slightly different functions in the cell. We have also found that copies 2 and 3 are activated by the lack of thiamine and that the Snz proteins physically interact with the thiamine biosynthesis Thi5 protein family. Whereas copy 1 is required for conditions in which B6 is essential for growth, copies 2 and 3 seem more related with B1 biosynthesis during the exponential phase. Copyright © 2002 John Wiley & Sons, Ltd.

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