Cut feather containing rachis as a sampling way for avian sexing
Article first published online: 13 FEB 2006
© 2006 Wiley-Liss, Inc.
Volume 25, Issue 4, pages 279–283, July/August 2006
How to Cite
Wang, L.-C., Chen, C.-T., Lee, H.-Y., Li, S.-H., Lir, J.-T., Chin, S.-C., Pu, C.-E. and Wang, C.-H. (2006), Cut feather containing rachis as a sampling way for avian sexing. Zoo Biol., 25: 279–283. doi: 10.1002/zoo.20083
- Issue published online: 10 AUG 2006
- Article first published online: 13 FEB 2006
- Manuscript Accepted: 30 AUG 2005
- Manuscript Received: 9 SEP 2004
- sex determination;
Sex determination of birds is important to ensure successful breeding strategies, especially for endangered species. Most birds are vulnerable to stress during handling, however, so obtaining a sufficient amount of genomic DNA (gDNA) while causing the least amount of harm is a critical issue. Avian gender can be determined based on different CHD1 gene intron sizes in W and Z sex chromosomes. We have compared various specimen sources and have found that the rachis segment of a feather is a good DNA source for determining sex. This indicates that plucking the whole feather is not necessary; a cut feather including the rachis is a superior method because it decreases stress on the examined birds and is accomplished easily. Zoo Biol. 0:1–5, 2006. © 2006 Wiley-Liss, Inc.