Failure of sister chromatid separation leads to formation of diplochromosomes in colcemid treated PtK1 cells

Authors

  • Sibdas Ghosh,

    1. Research Program IV, Division 0430, German Cancer Research Center, Im Neuenheimer Feld 280, D-69120 Heidelberg, FRG
    2. Department of Botany, University of Calcutta, Calcutta 700019, India. Tel: 49 6221 42 3403. Fax: 49 6221 42 3458.
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  • Neidhard Paweletz,

    Corresponding author
    1. Research Program IV, Division 0430, German Cancer Research Center, Im Neuenheimer Feld 280, D-69120 Heidelberg, FRG
      Research Program IV, Division 0430, German Cancer Research Center, Im Neuenheimer Feld 280, D-69120 Heidelberg, FRG
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  • Dieter Schroeter

    1. Research Program IV, Division 0430, German Cancer Research Center, Im Neuenheimer Feld 280, D-69120 Heidelberg, FRG
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Research Program IV, Division 0430, German Cancer Research Center, Im Neuenheimer Feld 280, D-69120 Heidelberg, FRG

Abstract

The origin of diplochromosomes has been traced in multinucleate rat kangaroo cells (PtK1) obtained after colcemid treatment. In these cells the diplochromosomes were shown to originate from restitution nuclei, indicating that they were formed due to the omission or failure of sister chromatid separation and not due to endoreduplication. In this context the mechanism of sister chromatid separation has been discussed. The independence of this mitotic event from other associated processes, such as chromosome condensation, nuclear envelope breakdown or spindle formation has been stressed.

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