Failure of centromere separation leads to formation of diplochromosomes in next mitosis in okadaic acid treated HeLa cells.
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1993 International Federation for Cell Biology
Cell Biology International
Volume 17, Issue 10, pages 949–952, October 1993
How to Cite
Ghosh, S., Paweletz, N. and Schroeter, D. (1993), Failure of centromere separation leads to formation of diplochromosomes in next mitosis in okadaic acid treated HeLa cells. Cell Biology International, 17: 949–952. doi: 10.1006/cbir.1993.1018
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Paper received 14.07.93. Revised paper accepted 04.09.93.
- Cited By
High concentrations of okadaic acid, sufficient to inhibit phosphatase 1 and 2A activities, induces formation of diplochromosomes in HeLa cells. It has been shown that this is due to a failure of sister chromatid separation in earlier mitosis in the presence of okadaic acid in the medium and not due to bypassing of mitosis (endoreduplication). Moreover, it has been demonstrated that the sister chromatid adherence does not depend on any under-replicated chromatin segment shared by the sister chromatids which might happen in okadaic acid induced premature mitosis, but due to the failure of the centromeres to separate at metaphase - anaphase transition. The role of phophatase 1 in sister chromatid separation has been discussed