Differential expression of adult type MHC in satellite cell cultures from regenerating fast and slow rat muscles.
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1993 International Federation for Cell Biology
Cell Biology International
Volume 17, Issue 11, pages 979–983, November 1993
How to Cite
Cantini, M., Fiorini, E., Catani, C. and Carraro, U. (1993), Differential expression of adult type MHC in satellite cell cultures from regenerating fast and slow rat muscles. Cell Biology International, 17: 979–983. doi: 10.1006/cbir.1993.1025
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Paper received 28.07.93. Revised paper accepted 09.10.93.
- Cited By
The local anaesthetic (Bupivacaine (1-n-butyl-DL-piperidine-2-carboxylic acid-2, 6-dimethyl anilide hydrochloride) has been used to induce myofiber damage (and thus satellite cells proliferation) and thereby represents a tool for increasing the yield of myoblasts from adult muscles. Replicating satellite cells were isolated by enzymatic dissociation from soleus (slow type) and tibialis anterior (fast type) muscles of adult rats, and categorized by the isoform (embryonic, fast and slow) of myosin heavy chain (MHC) expressed following myotube formation in a similar in vitro environment. According to light microscopic criteria, no morphological differences exist between the satellite cell cultures obtained from adult fast and slow muscles after Bupivacaine injection. On the other hand the derived myotubes express, beside the embryonic type, the peculiar myosin heavy chains which characterize the myosin pattern of the donor muscles.