Microtubules in dividing root cells of the conifer pinus radiata and the cycad zamia furfuracea


  • Larry C. Fowke

    1. Plant Cell Biology, Research School of Biological Sciences, Australian National University, Canberra, ACT, Australia.
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    • Permanent address: Department of Biology, University of Saskatchewan, Saskatoon, Sask., Canada, S7N 0W0.


Microtubules in dividing root cells of the gymnosperms Pinus radiata (conifer) and Zamia furfuracea (cycad) were examined using immunofluorescence techniques. Root tip squashes were prepared to visualize the 3-dimensional organization of microtubules in intact cells while sections of methacrylate embedded roots revealed microtubules in situ. Both species were characterized by well developed preprophase bands (PPB) of microtubules and highly focused spindle poles at prophase and anaphase. The metaphase spindle and telophase phragmoplast appeared typical of flowering plants.