Cytoskeletal changes and induction of embryogenesis in microspore and pollen cultures of Brassica napus L.
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1993 International Federation for Cell Biology
Cell Biology International
Volume 17, Issue 2, pages 153–168, February 1993
How to Cite
Hause, B., Hause, G., Pechan, P. and van Lammeren, A. A. M. (1993), Cytoskeletal changes and induction of embryogenesis in microspore and pollen cultures of Brassica napus L. Cell Biology International, 17: 153–168. doi: 10.1006/cbir.1993.1052
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Cited By
Microspores and pollen of Brassica napus were cultured under conditions leading to embryo formation. Concomitant changes in cytoskeletal configurations were analysed. The microfilamental cytoskeleton exhibited a loss of polarity in embryogenic cells but cytochalasin treatment revealed that microfilaments do not influence embryogenesis. Two embryogenic pathways started from microspores and were either characterized by turned division planes or by division when the nucleus was in the cell centre. In both cases microtubules clearly exhibited new arrangements and likely played a major role in newly induced symmetrical division. In pollen, embryogenic development started in the vegetative cell provided the generative cell was arrested near the pollen wall. The concomitant disappearance of defined microtubular arrays is likely to be responsible for the positioning of the cell.