Inhibition of an endogenous growth-related proteinase enhances the recovery of a negative growth regulator from cultured human cells.


Department of Biochemistry, University of Auckland, Private Bag 92019, Auckland, New Zealand.


The possibility that a growth-related proteinase may act by degrading a negative growth regulatory protein has been investigated. Proteinase inhibitors which inhibit the enzyme also enhance the accumulation of the growth regulator by human fibroblasts. The negative growth regulator shows a similar specificity of inhibition of cellular growth to inhibitors of the growth-related proteinase.