Transforming growth factor(TGF)-β1 induces leukemic cell-growth-promoting activity in fibroblast cells.
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1993 International Federation for Cell Biology
Cell Biology International
Volume 17, Issue 4, pages 433–440, April 1993
How to Cite
Komatsu, K., Nakamura, H. and Akedo, H. (1993), Transforming growth factor(TGF)-β1 induces leukemic cell-growth-promoting activity in fibroblast cells. Cell Biology International, 17: 433–440. doi: 10.1006/cbir.1993.1082
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Paper received 07.11.92. Revised paper accepted 14.12.92.
- Cited By
The production of the leukemic cell-growth-promoting factor (LGF) in TGF-β1-treated fibroblast cells was studied. BALB/c3T3 mouse fibroblast(3T3) cells cultured in Eagle's medium containing a low concentration of TGF-β1 (0.04-1 ng/ml) secreted 3-5 times more LGF than the cells cultured in the absence of TGF-β1. The amount of LGF secretion was dose-dependent on the concentration of post-cultured medium and time-dependent after the addition of TGF-β1. Similar findings were obtained in human diploid fibroblasts, WI-38 cells. LGF is a 18KD glycoprotein that is acid-stable but heat-unstable.