A cell cycle study of human mammary epithelial cells.
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1993 International Federation for Cell Biology
Cell Biology International
Volume 17, Issue 6, pages 565–571, June 1993
How to Cite
Larsson, O., Blegen, H., Wejde, J. and Zetterberg, A. (1993), A cell cycle study of human mammary epithelial cells. Cell Biology International, 17: 565–571. doi: 10.1006/cbir.1993.1100
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Paper received 18.05.92. Revised paper accepted 09.04.93.
- Cited By
The growth regulation of human mammary epithelial cells (HMEC) cultured in a growth factor/hormone-enriched (e.g. EGF, insulin) medium with bovine pituitary extract as the only undefined supplement was studied. The doubling times of the cultures, in which the cells appear in colonies, was 55-72 h, and a considerable intercolonial heterogenecity in proliferative activity could be demonstrated. However, every colony, irrespective of the size of the growth fraction, comprised a sub-population of rapidly growing cells which had a mean generation time of approximately 22 h. When insulin was removed from the culture medium, HMEC proliferation was inhibited. This growth inhibition was shown to be a result of a cell cycle-specific block.