The absence of confronting cisternae following thymidine-induced synchronization in HeLa cells
Version of Record online: 2 JAN 2013
© The Author(s) Journal compilation © 1993 International Federation for Cell Biology
Cell Biology International
Volume 17, Issue 7, pages 653–664, July 1993
How to Cite
Palisano, J. R. (1993), The absence of confronting cisternae following thymidine-induced synchronization in HeLa cells. Cell Biology International, 17: 653–664. doi: 10.1006/cbir.1993.1115
- Issue online: 2 JAN 2013
- Version of Record online: 2 JAN 2013
- Paper received 12.03.93. Revised paper accepted 29.05.93.
- Cited By
This is the first report of the elimination of confronting cisternae (CC), prominent organelles in dividing HeLa cells, upon experimental manipulation of the cells. CC are lost when a double thymidine block is employed to synchronize cell division. This observation is consistent with the hypothesis that the occurrence of CC in some fetal cells and in selected tumor cells depends on the speed at which the cells cycle through mitosis. If thymidine blocks DNA synthesis and arrests cells at the G1/S interface, then thymidine probably has an indirect effect on CC. This paper reports the effect of thymidine on the occurrence of CC and briefly discusses how inhibitors of membrane synthesis or microtubule polymerization may affect the occurrence of CC during mitosis.