Protein-free culture of VERO cells: a substrate for replication of human pathogenic viruses
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1993 International Federation for Cell Biology
Cell Biology International
Volume 17, Issue 9, pages 885–895, September 1993
How to Cite
Cinatl, J., Cinatl, J., Rabenau, H., Rapp, J., Kornhuber, B. and Doerr, H.-W. (1993), Protein-free culture of VERO cells: a substrate for replication of human pathogenic viruses. Cell Biology International, 17: 885–895. doi: 10.1006/cbir.1993.1152
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Paper received 06.05.93. Paper accepted 06.07.93.
- Cited By
A protein-free chemically defined medium designated PFEK-1 was developed for culture of VERO cells on polyvinyl formal (PVF) culture surface without serum or other macromolecular supplements. VERO cells proliferated in PFEK-1 medium on PVF surface to a similar extent as cells in serum-supplemented medium without previous adaptation from serum-containing conditions. The protein-free culture infected with coxsackievirus B4, herpes simplex virus types 1 and 2, measles virus and poliovirus types 1, 2 and 3 developed viral titers comparable to those found in conventionally grown cells. The results demonstrated that VERO cells in protein-free culture provide a sensitive substrate for the production of human pathogenic viruses which are not contaminated by serum or other protein factors usually added to a culture medium.