Transfected Chinese hamster ovary cells as a model system for cytokine immunocytochemistry and in situ hybridisation.
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1994 International Federation for Cell Biology
Cell Biology International
Volume 18, Issue 1, pages 55–61, January 1994
How to Cite
Mueller, R., Junker, U., Wagner, K., Heusser, C. and Bullock, G. (1994), Transfected Chinese hamster ovary cells as a model system for cytokine immunocytochemistry and in situ hybridisation. Cell Biology International, 18: 55–61. doi: 10.1006/cbir.1994.1007
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Paper received 28.08.93. Revised paper accepted 02.10.93.
- Cited By
The presence of cytokine producing cells is most easily revealed by techniques measuring the secreted cytokines in culture supernatants or body fluids. However, these techniques only measure the bulk cytokine release by a given, often mixed cell population. To demonstrate cytokine production at the single cell level, immunocytochemistry (ICC) and in situ hybridisation (ISH) are now widely used techniques. To establish these techniques, an easily accessible model system is needed which permits the evaluation of different ICC and ISH protocols. It can be used to demonstrate the specificity of the antibodies and may serve as a positive control for samples of unknown cytokine content. Here we propose the use of Chinese hamster ovary (CHO) cells transfected to express one specific cytokine as such a model system. Its usefulness is demonstrated by the characterisation of six monoclonal antibodies to human interleukin-4 and the establishment of two in situ hybridisation protocols.