Hypothermic preservation of hepatocytes on gelatin gels
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1994 International Federation for Cell Biology
Cell Biology International
Volume 18, Issue 11, pages 999–1008, November 1994
How to Cite
Evans, P. J. (1994), Hypothermic preservation of hepatocytes on gelatin gels. Cell Biology International, 18: 999–1008. doi: 10.1006/cbir.1994.1023
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Paper received 01.11.94. Paper accepted 07.11.94.
- Cited By
Hypothermic preservation of hepatocytes on gelatin gels allows hepatocytes to be stored for at least 9 days. The procedure is easy, inexpensive and does not require specialised equipment. The cells retain their morphology and are released as separate spherical entities by dissolving the gelatin layer at 37°. The recovered cells have an intact plasma membrane as judged by lactate dehydrogenase activity, attachment efficiency and subsequent monolayer formation in culture. Functional tests show that the cells recover quickly from the storage conditions. Rates of protein synthesis are maintained over a 6 day period and remain at 62% of the initial level on day 9. The liver specific, hormonal induction of the enzyme tyrosine aminotransferase is apparent throughout the culture period. In addition a phenotypic marker enzyme, γ-glutamyl transpeptidase, remained at basal levels.