Elongation factor 1α is a component of the subcortical actin bundles of characean algae.

Authors

  • David A. Collings,

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    1. Plant Cell Biology Group, Research School of Biological Sciences, Australian National University, GPO Box 475, Canberra, ACT2601, Australia
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  • Geoffrey O. Wasteneys,

    1. Plant Cell Biology Group, Research School of Biological Sciences, Australian National University, GPO Box 475, Canberra, ACT2601, Australia
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  • Masazumi Miyazaki,

    1. Department of Molecular Biology, School of Science, Nagoya University, Chikusa-ku, Nagoya, Japan.
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  • Richard E. Williamson

    1. Plant Cell Biology Group, Research School of Biological Sciences, Australian National University, GPO Box 475, Canberra, ACT2601, Australia
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ABSTRACT

Antibodies to elongation factor 1α (EF1α), a known 50 kDa actin-bundling protein in Dictyostelium, identified a protein in a whole cell extract of the characean alga Nitella pseudoflabellata that had an apparent molecular weight of 51 kDa. Indirect immunofluorescence microscopy revealed labelling by the EF1α antibodies of the subcortical actin bundles, even after the motile organelles of the endoplasm were removed by perfusion with ATP-containing solutions.

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