Effects of hypothermia on the survival and cryopreservation of minipig ileal cells and Chinese hamster ovary cells.
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1994 International Federation for Cell Biology
Cell Biology International
Volume 18, Issue 11, pages 1059–1065, November 1994
How to Cite
Kaeffer, B., Uriel, I. G. and Bottreau, E. (1994), Effects of hypothermia on the survival and cryopreservation of minipig ileal cells and Chinese hamster ovary cells. Cell Biology International, 18: 1059–1065. doi: 10.1006/cbir.1994.1029
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Paper received 03.09.94. Revised paper accepted 27.10.94.
- Cited By
Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypothermia pretreatment (2 days at 25°C and 3 hours recovery at 37°C) improved hamster cell survival to freeze-thaw damage (p < 0.01) but decreased the survival of 2 immortal pig ileal cell lines even though epithelioid IPI-2I cells were more tolerant to hypothermia than IPI-I fibroblasts. Epithelioid cells survived 3 days at 25°C with unaltered expression of cytokeratin-18 whereas colonies of fibroblasts did not survive more than a day at 25°C (p < 0.001). These results suggest that hypothermia-tolerance of pig ileal cell lines might differ according to cell lineage calling for further experiments on small intestinal primary cell culture.