Tumor necrosis factor alpha, a cytokine coregulating sugar uptake by cultured human synovial cells.
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1994 International Federation for Cell Biology
Cell Biology International
Volume 18, Issue 3, pages 159–164, March 1994
How to Cite
Hernvann, A., Souliac-Marc, C., Aussel, C. and Ekindjian, O. (1994), Tumor necrosis factor alpha, a cytokine coregulating sugar uptake by cultured human synovial cells. Cell Biology International, 18: 159–164. doi: 10.1006/cbir.1994.1056
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Paper received 18.10.93. Revised paper accepted 21.02.94.
- Cited By
Confluent cultures of osteoarthritic and rheumatoid human synovial cells were treated with human recombinant tumor necrosis factor alpha (TNF-α) The cytokine increased uptake of 2-deoxy-D-[1-3H]glucose (2-DOG) in a time- and concentrations-dependent manner. In synovial cells obtained from osteoarthritic patients (OA cells), the stimulation of 2-DOG uptake occurred 3 hours following addition of TNF-α (1 ng/ml) and was maximal by 24 hours Rheumatoid synovial cells (RA cells) appeared less sensitive to the cytokine: 2-DOG uptake stimulation was only significant after 6 hours of incubation. In both OA and RA cells, the effect was protein synthesis-dependent, and was not secondary to prostaglandin E2 synthesis or cell growth. Interteukin-1β was more efficient than TNF-α for 2-DOG uptake stimulation The two cytokines seemed to act in an additive manner.