Video enhanced laser scanning microscopic analysis of DNA comet formation.
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1994 International Federation for Cell Biology
Cell Biology International
Volume 18, Issue 3, pages 195–200, March 1994
How to Cite
Fairbairn, D. W., Rowe, K. C. and O'Neill, K. L. (1994), Video enhanced laser scanning microscopic analysis of DNA comet formation. Cell Biology International, 18: 195–200. doi: 10.1006/cbir.1994.1061
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Paper received 16.09.93 Paper accepted 18.02.94
- Cited By
Laser Scanning Microscopy is a sensitive tool that provides a unique method of analyzing biological systems. Coupled with the Single Cell Gel assay, it allows for accurate and reproducible detection of DNA strand breaks. An understanding of the theory of DNA comet formation is lacking. Using dexamethasone induced apoptosis in murine thymocytes as a model for double strand breaks, we used video enhanced laser scanning microscopy to evaluate the leading edge of DNA migration in the single cell gel assay. In this system, comet length increases significantly within the first thirty seconds of electrophoresis, the greatest increase in length is completed within the first minute, and the first two minutes are important in significant increases in DNA migration during DNA comet formation.