Aphidicolin induces myogenic differentiation in the human rhabdomyosarcoma cell line KFR.
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1994 International Federation for Cell Biology
Cell Biology International
Volume 18, Issue 4, pages 271–278, April 1994
How to Cite
Cinatl, J., Cinatl, J., Driever, P. H., Rabenau, H., Novak, M., Stefanik, M., Kornhuber, B. and Doerr, H.-W. (1994), Aphidicolin induces myogenic differentiation in the human rhabdomyosarcoma cell line KFR. Cell Biology International, 18: 271–278. doi: 10.1006/cbir.1994.1072
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Paper received 26.01.94. Revised paper accepted 08.04.94.
- Cited By
The effects of aphidicolin, a specific inhibitor of DNA polymerase α, on cell growth, DNA synthesis and myogenic differentiation in the human alveolar rhabdomyosarcoma cell line KFR were studied. The treatment with aphidicolin at 5 × 10−6 M concentration, which completely inhibited DNA synthesis and cell growth, induced morphological differentiation of small mononuclear cells to elongated, multinucleated (myotube-like) structures. The morphological differentiation was accompanied by the expression of skeletal muscle myosin; about 30% myosin-positive cells were observed after 14 days of treatment, compared to 2.3% in untreated cultures. The results showed that aphidicolin induces differentiation of human rhabdomyosarcoma cells and that multinucleated myotube-like elements may develop simply by cell fusion without cell division and DNA synthesis.