REVERSIBLE REARRANGEMENT OF VIMENTIN-TYPE INTERMEDIATE FILAMENTS IN CULTURED HUMAN SKIN FIBROBLASTS FROM PATIENTS WITH LYSOSOMAL STORAGE DISEASES
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1994 International Federation for Cell Biology
Cell Biology International
Volume 18, Issue 6, pages 647–654, June 1994
How to Cite
Tatiana, I. and German, W. (1994), REVERSIBLE REARRANGEMENT OF VIMENTIN-TYPE INTERMEDIATE FILAMENTS IN CULTURED HUMAN SKIN FIBROBLASTS FROM PATIENTS WITH LYSOSOMAL STORAGE DISEASES. Cell Biology International, 18: 647–654. doi: 10.1006/cbir.1994.1091
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Paper received 18.03.93. Revised paper accepted 29.04.94.
- Cited By
Immunofluorescence microscopy shows that unlike cytoplasmic microtubules (MT), vimentin-type intermediate filaments (IF) are collected into ring-shaped structures in affected fibroblasts. The altered IF organization could be observed in monolayers of polarized fibroblasts in the prolonged stationary growth phase and after replating - upon their initial spreading. Transition from a discoid to an extended cellular form is accompanied by centrifugal dislocation of ring-shaped IF structures towards the cell's active edge with gradual restoration of the radial fibrillar vimentin network. Spreading of affected cells occurred more slowly than that of control fibroblasts.