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Keywords:

  • Fibroblasts;
  • elastase-type protease;
  • Gelatinase A;
  • Matrix Metalloproteinase;
  • Interleukins

ABSTRACT

Gelatinase A and elastase type proteinase (Homsy, et al., 1988) present at plasma membranes of human skin fibroblasts (HSF) were separated by anion exchange chromatography on a DEAE Tris acryl M column. Elastase type proteinase (HSFE1) was able to convert 72 kDa progelatinase A to a lower 66 kDa M.W. active enzyme. Several cytokines (IL-1β, 1L4, IL6), interferon γ (IFN γ) and tumor growth factor β (TGFβ) were studied for their ability to modify the levels of those plasma membrane associated proteinases. Among these mediators, only IL-1β was found to enhance the amounts of HSF membrane-bound HSFE1 and Gelatinase A.