Effect of extracellular K+ on hatching and outgrowth of mouse blastocysts in vitro.
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1994 International Federation for Cell Biology
Cell Biology International
Volume 18, Issue 9, pages 897–901, September 1994
How to Cite
Jin, Z., Jin, M. and Roomans, G. M. (1994), Effect of extracellular K+ on hatching and outgrowth of mouse blastocysts in vitro. Cell Biology International, 18: 897–901. doi: 10.1006/cbir.1994.1127
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Paper received 09.06.94. Revised paper accepted 20.08.94.
- Cited By
The dependence of blastocyst development on the extracellular Na+/K+ ratio was investigated in an in vitro system. Hatching and outgrowth of mouse blastocysts was enhanced at Na+/K+ ratios between 3 and 10 compared to the ratio of about 25 typical for most culture media and serum. At a Na+/K+ ratio of 2, blastocyst hatching and outgrowth were inhibited. The requirement of blastocyst development for relatively high extracellular K+ concentrations agrees with the fact that K+ concentrations in oviduct and uterine secretions are higher than in serum. The findings can also be relevant in optimizing in vitro culturing techniques for blastocysts.