CRC Department of Medical Oncology, Christie Hospital NHS Trust, Manchester M20 9BX, U.K.
Changes in the nature of P-glycoprotein gene transcripts during the development of multidrug resistance in CHO cells.
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1995 International Federation for Cell Biology
Cell Biology International
Volume 19, Issue 1, pages 35–42, January 1995
How to Cite
Vickers, S. E. and Warr, J. R. (1995), Changes in the nature of P-glycoprotein gene transcripts during the development of multidrug resistance in CHO cells. Cell Biology International, 19: 35–42. doi: 10.1006/cbir.1995.1005
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Paper received 05.09.94. Paper accepted 05.12.94.
- Cited By
We have studied the nature of P-glycoprotein gene transcripts in multidrug resistant Chinese hamster ovary cell lines selected in vincristine. Using a novel, 35S based, reverse transcription polymerase chain reaction assay, we have shown that there is a considerable increase in the level of pgp2 gene transcripts, with levels rising to a hundred fold those observed in sensitive control cells. The level of pgp1 gene transcripts was raised five fold. The levels of pgp3 gene transcripts were close to the limits of detection by the assay, but were increased in the more highly resistant cell lines. An increase in the size of the P-glycoprotein gene transcript was also observed in some of the resistant cell lines.