Role of feeder cells in spreading and cytoskeleton organization of newborn rat keratinocytes.
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1995 International Federation for Cell Biology
Cell Biology International
Volume 19, Issue 1, pages 59–64, January 1995
How to Cite
Gorelik, J. V., Blinova, M. I., Diakonov, I. A., Kukhareva, L. V. and Pinaev, G. P. (1995), Role of feeder cells in spreading and cytoskeleton organization of newborn rat keratinocytes. Cell Biology International, 19: 59–64. doi: 10.1006/cbir.1995.1008
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Paper received 10.08.94. Revised paper accepted 20.12.94.
- Cited By
We studied the effect of feeder cells (fibroblasts) and a mixture of the extracellular matrix components, Matrigel, on spreading and cytoskeleton organization of newborn rat keratinocytes (REK). REK formed lamellipodia on being plated together with feeder cells and on the Matrigel as a substrate whereas the same REK plated alone on a plastic surface formed filopodia. REK lamellipodia formation in co-cultures depended on the fibroblast addition time. Although conditioned medium from fibroblast cultures was not enough to induce lamellipodia, the extracellular matrix left after fibroblast removal was as effective as Matrigel. Our results indicate that lamellipodia formation seems to depend on the factor(s) secreted by fibroblasts and associated with the extracellular matrix.