Differences in the motility of Amoeba proteus isolated fragments are determined by F-actin arrangement and cell nucleus presence
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1995 International Federation for Cell Biology
Cell Biology International
Volume 19, Issue 10, pages 847–854, October 1995
How to Cite
Greȩbecka, L., Pomorski, P. and Łopatowska, A. (1995), Differences in the motility of Amoeba proteus isolated fragments are determined by F-actin arrangement and cell nucleus presence. Cell Biology International, 19: 847–854. doi: 10.1006/cbir.1995.1020
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
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Isolated fragments produced by bisection of Amoeba proteus differ by their position in the original cell and by the presence or absence of the cell nucleus. Immediately after the operation, both types of anterior fragments preserve the former motory polarity, and do not interrupt locomotion. In the same time, all posterior fragments stop, round up and fail to react stimuli. In the second phase of experiment, these anterior fragments, which had no nucleus ceased to move, whereas the nucleated posterior ones resumed locomotion.
It was demonstrated, that the behaviour of a fragment is primarily determined by the peripheral F-actin distribution, which is different depending on the origin of the fragment either from the anterior or from the posterior cell region. Later, the "inherited" F-actin distribution may be stabilized or reorganized in the presence of the nucleus, or desorganized in its absence.