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ABSTRACT

Highly purified plasma membrane was prepared from etiolated zucchini hypocotyls, developing pea cotyledons and suspension-cultured soybean cells by phase partitioning and sucrose density gradient centrifugation. The brain β-adaptin monoclonal antibody B1/M6, which recognizes a similar adaptin in zucchini clathrin coated vesicles, cross-reacted with an electrophoretically identical polypeptide in the plant plasma membrane fractions. Immunogold labelling of thin sections of soybean cells confirmed the presence of the β-adaptin at the plasma membrane. The presence of this adaptin at the Golgi apparatus could not be demonstrated unequivocally.