Production of human immunodeficiency virus by chronically infected cells grown in protein-free medium.

Authors

  • Claudia Pintér,

    1. Dipartimento di Biologia e Genetica per le Scienze Mediche, Università degli Studi di Milano, Via G. B. Viotti 3/5, 20133 Milano
    2. DIBIT San Raffaele, Milano, Italy.
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  • Antonio G. Siccardi,

    1. Dipartimento di Biologia e Genetica per le Scienze Mediche, Università degli Studi di Milano, Via G. B. Viotti 3/5, 20133 Milano
    2. DIBIT San Raffaele, Milano, Italy.
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  • Alberto Clivio

    Corresponding author
    1. Dipartimento di Biologia e Genetica per le Scienze Mediche, Università degli Studi di Milano, Via G. B. Viotti 3/5, 20133 Milano
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Correspondence author

Abstract

A human T cell line chronically infected with Human Immunodeficiency Virus (HIV) has been adapted to grow in a chemically defined, protein-free medium. Virus particles are produced at rates comparable to those of serum-supplemented cultures; virus preparations free of undesirable proteins can be produced in preparative amounts by simple ultrafiltration procedures and cell culture supernatants can be used as such for the preparation of ELISA solid phases. This material has been used very conveniently for studies concerning characterization of antibodies against HlV-specific proteins, interaction of HIV with complement components and inclusion of human cell-derived proteins into virions; we propose its use as a powerful tool for the structural as well as functional analysis of the virus particle itself.

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