Regulation of fibronectin expression by PDGF-BB And IGF-I in cultured rat thoracic aortic adventitial fibroblasts.

Authors

  • Jing Zhang,

    1. Department of Physiology, Uniformed Services University of the Health Science, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
    Search for more papers by this author
  • Chu-shek Lo

    Corresponding author
    1. Department of Physiology, Uniformed Services University of the Health Science, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
    Search for more papers by this author

Correspondence author

Abstract

Regulation of fibronectin (FN) by Platelet-derived Growth Factor-BB (PDGF-BB) and Insulin-like Growth Factor-I (IGF-I) in cultured rat thoracic aortic adventitial fibroblasts were examined. PDGF-BB enhances FN mRNA levels in a time and concentration-dependent fashion. The effect of IGF-I on PDGF-BB-induced FN levels was also examined. There was a larger increase in FN mRNA levels (4-fold) in the cells treated with both IGF-I and PDGF-BB than with either IGF-I (2-fold) or PDGF-BB (2-fold) alone. The effects of PDGF-BB and IGF-I on FN levels were examined. There was a larger increase in FN levels (135%) in the cells treated with both PDGF-BB and IGF-I than with either PDGF-BB (43%) or IGF-I (45%) alone. Regulation of adventitial fibroblast FN may lead to blood vessel diseases and/or angiogenesis.

Ancillary