Gene expression and protein distribution of inter-α-trypsin inhibitor in three human hepatoma cell lines.

Authors

  • Antoine Héron,

    1. INSERM Unité 295, Faculté de Médecine-Pharmacie de Rouen, B.P. 97, Avenue de l'Université, F-76803 St Etienne Rouvray Cedex, FRANCE and ERPUR (Etudes et Recherches en Pneumologie de l'Université de Rouen).
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  • Hélène Borghi,

    1. INSERM Unité 295, Faculté de Médecine-Pharmacie de Rouen, B.P. 97, Avenue de l'Université, F-76803 St Etienne Rouvray Cedex, FRANCE and ERPUR (Etudes et Recherches en Pneumologie de l'Université de Rouen).
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  • Aleth Callé,

    1. INSERM Unité 295, Faculté de Médecine-Pharmacie de Rouen, B.P. 97, Avenue de l'Université, F-76803 St Etienne Rouvray Cedex, FRANCE and ERPUR (Etudes et Recherches en Pneumologie de l'Université de Rouen).
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  • Jeannette Bourguignon,

    Corresponding author
    1. INSERM Unité 295, Faculté de Médecine-Pharmacie de Rouen, B.P. 97, Avenue de l'Université, F-76803 St Etienne Rouvray Cedex, FRANCE and ERPUR (Etudes et Recherches en Pneumologie de l'Université de Rouen).
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  • Maryam Diarra-Mehrpour,

    1. INSERM Unité 295, Faculté de Médecine-Pharmacie de Rouen, B.P. 97, Avenue de l'Université, F-76803 St Etienne Rouvray Cedex, FRANCE and ERPUR (Etudes et Recherches en Pneumologie de l'Université de Rouen).
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  • Jean-Pierre Martin,

    1. INSERM Unité 295, Faculté de Médecine-Pharmacie de Rouen, B.P. 97, Avenue de l'Université, F-76803 St Etienne Rouvray Cedex, FRANCE and ERPUR (Etudes et Recherches en Pneumologie de l'Université de Rouen).
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  • Richard Sesboüé

    1. INSERM Unité 295, Faculté de Médecine-Pharmacie de Rouen, B.P. 97, Avenue de l'Université, F-76803 St Etienne Rouvray Cedex, FRANCE and ERPUR (Etudes et Recherches en Pneumologie de l'Université de Rouen).
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To whom correspondence should be addressed.

Abstract

In standard culture conditions, three human hepatoma cell lines, Hep3B, PLC/PRF/5 and HepG2, were characterised by a predominant transcription of only two (H2 and L) among the four genes involved in the synthesis of inter-α-trypsin inhibitor (ITI)-related proteins. Pulse-chase experiments followed by immunoprecipitation with specific anti-L and anti-H ITI antisera showed that the proteins synthesised displayed a restricted L and/or H2 antigenic reactivity. Furthermore, while Hep3B and PLC/PRF/5 lines only synthesised ITI precursors (mainly the L-form), HepG2 cells were able to secrete an ITI-like protein. Immunocytochemical analyses substantiated these results with uneven distribution of heavy and light-chain polypeptide reactivity among the cells. The use of hepatoma cell models for the study of protein synthesis and assembly must therefore be considered cautiously.

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