CHARACTERIZATION OF FORCED SUSPENSION CULTURE OF SPONTANEOUSLY TRANSFORMED MOUSE FIBROBLASTS (B-6)

Authors

  • TADAO MATSUHISA,

    1. Department of Cell Biology, The Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3, Nakamichi, Higashinari, Osaka, 537, Japan
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  • KOUICHI TAKEMURA

    1. Department of Electron Microscopy, The Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3, Nakamichi, Higashinari, Osaka, 537, Japan
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Abstract

Mouse fibroblasts (B-6) were cultured on agar-coated dishes. After cells grew for 2–3 generations relatively rapidly in suspension, they began to grow very slowly (stationary phase). Electron microscopic studies showed that cells in a stationary phase developed intracellular organella: membranous structures (endoplasmic reticulum and Golgi apparatus) became manifest and the number of mitochondria increased. The specific activities of succinic-cytochrome c reductase and 5′-nucleotidase were three and five times higher, respectively, than those of cells on the dish.

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