HYPERTHERMIC STRESS AFFECTS GLUCOCORTICOID RECEPTOR-MEDIATED TRANSCRIPTION IN RAT LIVER

Authors

  • STOJKO VIDOVIĆ,

    1. School of Medicine, University of Banja Luka, Department of Biology and Human Genetics, 29. Novembra 142, 11060, Belgrade, Yugoslavia
    Search for more papers by this author
  • ALEKSANDRA ČVORO,

    1. Institute for Biological Research, Department of Biochemistry and Molecular Biology, 29. Novembra 142, 11060, Belgrade, Yugoslavia
    Search for more papers by this author
  • JADRANKA DUNDJERSKI,

    1. Institute for Biological Research, Department of Biochemistry and Molecular Biology, 29. Novembra 142, 11060, Belgrade, Yugoslavia
    Search for more papers by this author
  • DIVNA TRAJKOVIĆ,

    1. Institute for Biological Research, Department of Biochemistry and Molecular Biology, 29. Novembra 142, 11060, Belgrade, Yugoslavia
    Search for more papers by this author
  • GORDANA MATIĆ

    Corresponding author
    1. Institute for Biological Research, Department of Biochemistry and Molecular Biology, 29. Novembra 142, 11060, Belgrade, Yugoslavia
    Search for more papers by this author

To whom correspondence should be addressed.

Abstract

Binding capacity of the cytoplasmic and nuclear glucocorticoid receptor (GR) and the activity of tyrosine aminotransferase (TAT) were examined in the liver of intact and adrenalectomized rats exposed to 41°C whole body hyperthermic stress. In glucocorticoid-deprived animals, stress-induced decrease in the cytoplasmic steroid binding was followed by parallel increases in its nuclear binding and TAT activity, suggesting a stimulation of TAT gene transcription by the GR in the absence of the ligand. In intact animals, however, a diminution of the steroid binding in the cytosol, its unchanged nuclear binding and an impairment of TAT activity were observed upon the stress. The results imply that stress could elicit different structural or functional alterations of unligandedvsliganded GR.

Ancillary