MOSQUITO LARVAE EXTRACT INDUCES MITOTIC RATE ALTERATIONS OF MICE TONGUE KERATINOCYTES ALONG A CIRCADIAN RHYTHM PERIOD

Authors

  • J. R. RONDEROS,

    Corresponding author
    1. Catedra de Histologia y Embriologia ‘B’, Fac. de Cs. Medicas, Universidad Nacional de La Plata, Calle 60 y 120 (1900), La Plata, Argentina
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  • F. L. RICCILLO,

    1. Catedra de Histologia y Embriologia ‘B’, Fac. de Cs. Medicas, Universidad Nacional de La Plata, Calle 60 y 120 (1900), La Plata, Argentina
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  • A. OTTINO

    1. Catedra de Histologia y Embriologia ‘B’, Fac. de Cs. Medicas, Universidad Nacional de La Plata, Calle 60 y 120 (1900), La Plata, Argentina
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Abstract

It has been demonstrated that the crude extract of mosquito larvae alters the mitotic rate of several mouse cell populations of young growing mice (25±1 days old). Furthermore, the dialysed fraction of the extract inhibited proliferation of hepatocytes from hepatectomized adult male mice (90 days old). Sampling during the period between 16 and 24 h after treatment (when mitotic peak normally occurs) shows an inhibiting effect on the G1/S interphase, whereas evaluation during the dark phase of the circadian rhythm period (i.e. 4 to 12 h after treatment) shows an increment of the mitotic rates suggesting a probable effect at G2/M restriction point. In the present paper we report the effect of the mosquito larvae crude extract on the proliferative activity of tongue keratinocytes along a circadian rhythm period. Treatments were intra-peritoneally applied at 16/00 Time of Day/Time Post Injection and mice were killed at 00/08, 04/12, 08/16, 12/20 and 16/24 TD/TPI. As other cell populations previously analysed, the mitotic rate of tongue keratinocytes of extract receivers was significantly increased during night (when S phase normally occurs) and inhibited during the 08/16 to 16/24 period.

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