PRODUCTION OF BIOLOGICALLY ACTIVE HUMAN RELA (p65) IN BACULOVIRUS-INFECTED INSECT CELLS

Authors


To whom correspondence should be addressed.

Abstract

A recombinant baculovirus was constructed to express a cDNA encoding RelA (p65), a member of the NF-κB/Rel family of proteins. Infection of Spodoptera frugiderda insect cells with the recombinant baculovirus resulted in the production of the biologically active protein as measured by immunoblotting using RelA-specific antisera and by electrophoretic mobility shift assays. The recombinant protein bound specifically to an oligonucleotide containing the NF-κB consensus motif but not to that containing the unrelated Oct-1 consensus motif. Thus insect cell-derived RelA possess properties similar to the native protein and may be used in physical, biochemical, and pharmacological studies.

Ancillary