ALTERATIONS IN IMMUNOGLOBULIN SYNTHESIS DURING ADRIAMYCIN-INDUCED APOPTOSIS IN MOUSE HYBRIDOMA CELLS

Authors

  • M. B. MELIKSETIAN,

    1. Institute of Cytology, Russian Academy of Sciences, Tikhoretsky Prospect 4, 194064, St Petersburg, Russian Federation
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  • T. K. DAVTYAN,

    1. Institute of Epidemiology and Virology, Ministry of Health of Armenia, G. Kochar Street 32a, 375009, Yerevan, Republic of Armenia
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  • I. V. CHIRKOWA,

    1. Institute of Cytology, Russian Academy of Sciences, Tikhoretsky Prospect 4, 194064, St Petersburg, Russian Federation
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  • YU.T. ALEXANYAN,

    1. Institute of Epidemiology and Virology, Ministry of Health of Armenia, G. Kochar Street 32a, 375009, Yerevan, Republic of Armenia
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  • T. N. IGNATOVA

    1. Institute of Cytology, Russian Academy of Sciences, Tikhoretsky Prospect 4, 194064, St Petersburg, Russian Federation
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    • Present address: Cancer Center, University of Illinois at Chicago, IL 60607-7173, U.S.A.


Abstract

Changes in the immunoglobulin synthesis in mouse hybridoma 1F7cell line following exposure to the anticancer drug adriamycin were studied. It was shown that adriamycin in concentrations up to 0.1μg/ml causes dose-dependent reversible enhancement of immunoglobulin heavy γ2bchain synthesis, and to a lesser extent, κ light chain synthesis. Enhancement of γ2bheavy chain synthesis accompanies an increase in Ig γ2bmRNA levels. Enhancement of DNA-binding activity of another B-cell specific protein, octamer transcription factor Oct-2 was also observed. Cell cycle analysis revealed accumulation of the ADR-treated cells in G2/M phase of cell cycle. These changes in hybridoma cells preceded the onset of internucleosomal DNA fragmentation and appearance of morphological characteristics of apoptosis. These findings suggest, that differentiation-related processes occur during the latent phase of apoptosis induced by adria-mycin in mouse hybridoma cells.

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