MONOCLONAL ANTIBODY RAISED AGAINST HUMAN MITOTIC CYCLIN B1IDENTIFIES CYCLIN B-LIKE MITOTIC PROTEINS IN SYNCHRONIZED ONION (ALLIUM CEPA L.) ROOT MERISTEM

Authors

  • SADHAN K. CHAUDHURI,

    1. Centre of Advanced Study in Botany, Cell Biology Unit, University of Calcutta, 35, Ballygunge Circular Road, Calcutta, 700019, India
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  • SIBDAS GHOSH

    1. Centre of Advanced Study in Botany, Cell Biology Unit, University of Calcutta, 35, Ballygunge Circular Road, Calcutta, 700019, India
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Abstract

Cyclin B-like mitotic proteins have been detected in synchronized Allium cepa L. root tip cells by using mouse monoclonal anti-cyclin B1antibody raised against human cyclin B1Immunoblot shows two closely placed isoforms of cyclin B-like proteins having an apparent molecular weight around 54 kDa. In vivo [35S]-methionine labelling followed by immunoprecipitation and autoradiography indicates that cyclin B-like proteins are mainly synthesized in the G2 phase of the cell cycle and destroyed in late mitosis. Immunoblotting data depict that the level of cyclin B-like proteins reaches the maximum at the late G2 to early M phase; and it becomes degraded in the late hours of mitosis. Moreover, the cyclin B isoforms are stabilized in colchicine-arrested metaphase cells as already reported in animal cells.

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