INFLUENCE OF SUCCESSIVE AND COMBINED ULTRAVIOLET A AND B IRRADIATIONS ON MATRIX METALLOELASTASES PRODUCED BY HUMAN DERMAL FIBROBLASTS IN CULTURE

Authors

  • C. KUT,

    1. Biochemistry Department, Faculty of Dental Surgery, University René Descartes Paris V, 1 rue M. Arnoux, 92120, Montrouge
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  • W. HORNEBECK,

    1. SIME UPRESA CNRS 6021, IFR 53 Biomolecules, Laboratory of Biochemistry, 51 rue Cognacq Jay, 51095, Reims Cedex
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  • N. GROULT,

    1. Biochemistry Department, Faculty of Dental Surgery, University René Descartes Paris V, 1 rue M. Arnoux, 92120, Montrouge
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  • G. REDZINIACK,

    1. Parfums Christian Dior, 45804 St Jean de Braye, France
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    • Vegetal Biology Laboratory, Yves Rocher, 3 allée de Grenelle, 91250 Issy les Moulineaux, France

  • G. GODEAU,

    1. Biochemistry Department, Faculty of Dental Surgery, University René Descartes Paris V, 1 rue M. Arnoux, 92120, Montrouge
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  • B. PELLAT

    1. Biochemistry Department, Faculty of Dental Surgery, University René Descartes Paris V, 1 rue M. Arnoux, 92120, Montrouge
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    • Biochemistry Department, Faculty of Dental Surgery, University René Descartes Paris V, 1 rue M. Arnoux, 92120, Montrouge


Abstract

To study the cumulative influence of UV irradiations on skin matrix alterations, human skin fibroblasts were irradiated successively three-fold, at 24h intervals, with UVA (3×5J/cm2), UVB (3×8mJ/cm2), UVA plus UVB (3×5J/cm2and 3×8mJ/cm2) and the levels of 92kDa gelatinase (pro-MMP9), 72kDa gelatinase (pro-MMP2) and plasma-membrane elastase type protease were determined, following subsequent 24-h culture in 10% serum-containing medium. UV irradiations had only minor influence (1.4-fold increase for UVB) on secreted levels of pro-MMP2and decreased the amount of plasma membrane elastase produced by cells. It did however, for UVA and UVB alone, induce a significant increase of 66kDa activated MMP2production: 2.5- and 1.7-fold respectively. Such enhancement was not observed when combined irradiations were administered. UV exposure possessed a much higher influence on pro-MMP9secretion by dermal fibroblast enhancing enzyme levels by 2.5-, 6.5- and 5-fold for UVA, UVB and UVA+UVB, respectively.

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