EFFECTS OF EXPOSURE TO ELECTROMAGNETIC RADIATION AT 835 MHz ON GROWTH, MORPHOLOGY AND SECRETORY CHARACTERISTICS OF A MAST CELL ANALOGUE, RBL-2H3

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Abstract

A mast cell line, RBL-2H3, was exposed to 835MHz for 20 minutes, three times per day for 7 days at a power density of 8.1±3mW/cm2From day 4 onwards, it was observed that the rate of DNA synthesis and cell replication increased, that actin distribution and cell morphology became altered, and the amount of β-hexosaminidase (a marker of granule secretion) released in response to a calcium ionophore was significantly enhanced, in comparison to unexposed cultures. There were no effects seen on levels of cytoskeletal protein synthesis or of beta-actin mRNA. Morphological changes persisted following subculture for at least 7 days in the absence of further exposure. It is hypothesized that effects of exposure to an electromagnetic field at 835MHz may be mediated via a signal transduction pathway.

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