PPRAS1 PPRAS2ANDPPRAP1GENES, MEMBERS OF ARASGENE FAMILY FROM THE TRUE SLIME MOLDPHYSARUM POLYCEPHALUMARE DEVELOPMENTALLY REGULATED

Authors


To whom correspondence should be addressed.

Abstract

The expression patterns of two truerasgenes,Ppras1andPpras2and onerapgene,Pprap1were examined in fourPhysarum polycephalumdevelopmental stages: uninucleate amoebae, plasmodia (multinucleate syncytia), spherules (a vegetative, dormant stage) and fruiting bodies.Ppras1andPprap1are expressed in all stages examined with the maximum levels of their transcripts in amoebae and fruiting bodies, respectively, and the minimum levels in plasmodia, whereas thePpras2transcript is only detectable in amoebae and fruiting bodies. The results obtained indicate thatP. polycephalumis an organism possessing a developmentally regulatedrasgene family and presents a convenient system to study the role ofras/rapgenes in control of growth and differentiation of lower eukaryotic organisms.

Ancillary