IL-1 MODIFIES SYSTEM A TRANSPORT ACTIVITY IN HUMAN RHEUMATOID SYNOVIAL CELLS

Authors

  • VALÉRIE LE MAIRE,

    Corresponding author
    1. Laboratoire de Biochimie Cellulaire de l'Inprimary_articlemmation, Université Paris Sud, 5 rue J.B. Clément, 92290, Châtenay-Malabry
    2. Laboratoire de Biochimie A, Hopital Cochin, 27 rue du Fg. St Jacques, 75014, Paris
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  • ALAIN HERNVANN,

    1. Laboratoire de Biochimie Cellulaire de l'Inprimary_articlemmation, Université Paris Sud, 5 rue J.B. Clément, 92290, Châtenay-Malabry
    2. Laboratoire de Biochimie A, Hopital Cochin, 27 rue du Fg. St Jacques, 75014, Paris
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  • HERVÉ LE MARECHAL,

    1. Laboratoire de Biochimie A, Hopital Cochin, 27 rue du Fg. St Jacques, 75014, Paris
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  • OHVANESSE G. EKINDJIAN,

    1. Laboratoire de Biochimie Cellulaire de l'Inprimary_articlemmation, Université Paris Sud, 5 rue J.B. Clément, 92290, Châtenay-Malabry
    2. Laboratoire de Biochimie A, Hopital Cochin, 27 rue du Fg. St Jacques, 75014, Paris
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  • CHRISTIAN AUSSEL

    1. Laboratoire de Biochimie Cellulaire de l'Inprimary_articlemmation, Université Paris Sud, 5 rue J.B. Clément, 92290, Châtenay-Malabry
    2. Laboratoire de Biochimie A, Hôpital Saint-Antoine, 184 rue du Fg. St Antoine, 75012, Paris, France
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To whom correspondence and reprint requests should be addressed.

Abstract

Given the importance of interleukin-1 in both rheumatic diseases and the modulation of cell metabolic activities, we studied the action of this cytokine on the neutral amino acid transport A system on rheumatoid synovial cells. In these cells IL-1 (1ng/ml) induced amino transport stimulation from 5min to 5h. This effect was obtained only after a starvation period. No concentration-related effect was found for IL-1-stimulated MeAIB uptake, and the IL-1-mediated MeAIB uptake stimulation is independent of protein synthesis. Neosynthesis or post-translational maturation of protein transport is a prerequisite for obtaining this effect. In conclusion, rheumatoid synovial cells exhibit a higher sensitivity for IL-1 than osteoarthritic ones, probably related to their intense metabolic activity.

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