THE EFFECT OF ENVIRONMENTAL ANEUPLOIDY-INDUCING AGENTS ON THE MICROTUBULE ARCHITECTURE OF MITOTIC MERISTEMATIC ROOT CELLS INHORDEUM VULGARE

Authors

  • G. VOUTSINAS,

    1. National Research Center ‘Democritus’ Institute of Biology, P.O. Box 60228, 15310, Aghia Paraskevi Attikis, Athens, Greece
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    • Present Address: Laboratory of Biology, School of Medicine, University of Patras, 26110 Patras, Greece

  • F.E ZARANI,

    1. National Research Center ‘Democritus’ Institute of Biology, P.O. Box 60228, 15310, Aghia Paraskevi Attikis, Athens, Greece
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  • A. KAPPAS

    Corresponding author
    1. National Research Center ‘Democritus’ Institute of Biology, P.O. Box 60228, 15310, Aghia Paraskevi Attikis, Athens, Greece
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To whom correspondence should be addressed.

Abstract

Microtubules are the prime components involved in chromosomal segregation, their functional accuracy ensuring maintenance of the normal karyotype in the progeny. Chemically-induced disruption of microtubules during mitosis can lead to aneuploidy. In this study, seven environmental chemicals, i.e. cadmium chloride (CD), econazole nitrate (EZ), benomyl (BM), thiabendazole (TB), griseofulvin (GF), thimerosal (TM) and hydroquinone (HQ), were tested for their ability to induce microtubule disruption in mitotic meristematic root cells of the higher plantHordeum vulgarewith the use of anti-tubulin indirect immunofluorescence microscopy. All chemicals tested in this study, with the exception of TB and HQ, produced modifications in the morphology of microtubule organization and reduced the fidelity of the spindle apparatus inHordeum vulgare

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