COMPARISON OF THE GROWTH PATTERNS AND MORPHOLOGICAL CHARACTERISTICS OF MECHANICALLY AND ENZYMATICALLY ISOLATED FALLOPIAN TUBE EPITHELIAL CELLS

Authors

  • Saeeda Sattar,

    1. Faculty of Science, School of Life Sciences, Kingston University, Penrhyn Road, Kingston-Upon-Thames, Surrey, KT1 2EE, U.K.
    Search for more papers by this author
  • Babar Abbas,

    1. Faculty of Science, School of Life Sciences, Kingston University, Penrhyn Road, Kingston-Upon-Thames, Surrey, KT1 2EE, U.K.
    Search for more papers by this author
  • Lucy Jones,

    1. Faculty of Science, School of Life Sciences, Kingston University, Penrhyn Road, Kingston-Upon-Thames, Surrey, KT1 2EE, U.K.
    Search for more papers by this author
  • Ertan Saridogan,

    1. Academic Department of Obstetrics, Gynaecology and Reproductive Physiology, St Bartholomew's & The Royal London School of Medicine and Dentistry, Whitechapel, London, E1 1BB, U.K.
    Search for more papers by this author
  • Tariq Mahmood,

    1. Academic Department of Obstetrics, Gynaecology and Reproductive Physiology, St Bartholomew's & The Royal London School of Medicine and Dentistry, Whitechapel, London, E1 1BB, U.K.
    Search for more papers by this author
  • Jayant Mehta,

    1. Academic Department of Obstetrics, Gynaecology and Reproductive Physiology, St Bartholomew's & The Royal London School of Medicine and Dentistry, Whitechapel, London, E1 1BB, U.K.
    Search for more papers by this author
  • Ovrang Djahanbakhch

    Corresponding author
    1. Academic Department of Obstetrics, Gynaecology and Reproductive Physiology, St Bartholomew's & The Royal London School of Medicine and Dentistry, Whitechapel, London, E1 1BB, U.K.
    Search for more papers by this author

Academic Department of Obstetrics, Gynaecology and Reproductive Physiology, St Bartholomew's & The Royal London School of Medicine and Dentistry, Whitechapel, London, E1 1BB, U.K.

Abstract

This study set out to compare the growth patterns and morphological characteristics of human fallopian tube epithelial cells isolated: (1) mechanically; and (2) enzymatically. Cells were cultured in medium supplemented with fetal bovine serum and antibiotics and their epithelial nature was established by immunocytochemistry for cytokeratins. Primary cultures were polygonal in shape with centrally located nuclei, irrespective of the isolation method. Cells isolated enzymatically exhibited a higher growth rate, but the survival rate was poor after more than 2–3 passages. Mechanical isolation gave a lower yield of cells, but had a higher survival rate when sub-cultured, even beyond 8 passages. Thus, mechanically isolated cells might be useful for longer term cultures, whereas enzymatically isolated cells are best only for short-term work.

Ancillary