CALCIUM LEVELS DURING CELL CYCLE CORRELATE WITH CELL FATE OF DICTYOSTELIUM DISCOIDEUM
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1999 International Federation for Cell Biology
Cell Biology International
Volume 23, Issue 6, pages 399–405, June 1999
How to Cite
Saran, S. (1999), CALCIUM LEVELS DURING CELL CYCLE CORRELATE WITH CELL FATE OF DICTYOSTELIUM DISCOIDEUM. Cell Biology International, 23: 399–405. doi: 10.1006/cbir.1999.0379
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 16 March 1998; accepted 5 March 1999
- Cited By
- Dictyostelium cell cycle;
- cell differentiation
Levels of intracellular calcium, (Ca2+)i, from different stages of cell cycle of Dictyostelium discoideum were monitored using the fluorescent Ca2+-sensitive dye, Indo 1. Combinations of Ca2+-ionophore (A23187) and Ca2+-chelator (EGTA) resulted in the inhibition of progression of cell cycle. This delay was caused due to block in G2/MS phase transition of the cell cycle. Rescue of the cell cycle progression was made with 0.5m m of exogenous Ca2+. High (Ca2+)ilevels overlapped with the S-phase, of the cell cycle.
Results indicate that a high (Ca2+)ilevel during S-phase is not required for cell cycle progression but for cell-type choice mechanism at the onset of starvation, and these cells tend to follow the prestalk pathway.