THE NATIVE MEMBRANE FUSION MACHINERY IN CELLS

Authors

  • Eun-Hwan Jeong,

    1. Department of Surgery & Biomedical Engineering Program, Yale University School of Medicine, New Haven, CT, 06510, U.S.A.
    2. Cell Biology, Yale University School of Medicine, New Haven, CT, 06510, U.S.A.
    Search for more papers by this author
  • Paul Webster,

    1. Department of Surgery & Biomedical Engineering Program, Yale University School of Medicine, New Haven, CT, 06510, U.S.A.
    2. Cell Biology, Yale University School of Medicine, New Haven, CT, 06510, U.S.A.
    Search for more papers by this author
  • Chau Q. Khuong,

    1. Department of Surgery & Biomedical Engineering Program, Yale University School of Medicine, New Haven, CT, 06510, U.S.A.
    2. Cell Biology, Yale University School of Medicine, New Haven, CT, 06510, U.S.A.
    Search for more papers by this author
  • Abdus A. K. M. Sattar,

    1. Department of Surgery & Biomedical Engineering Program, Yale University School of Medicine, New Haven, CT, 06510, U.S.A.
    2. Cell Biology, Yale University School of Medicine, New Haven, CT, 06510, U.S.A.
    Search for more papers by this author
  • Mylan Satchi,

    1. Department of Surgery & Biomedical Engineering Program, Yale University School of Medicine, New Haven, CT, 06510, U.S.A.
    2. Cell Biology, Yale University School of Medicine, New Haven, CT, 06510, U.S.A.
    Search for more papers by this author
  • Bhanu P. Jena

    Corresponding author
    1. Department of Surgery & Biomedical Engineering Program, Yale University School of Medicine, New Haven, CT, 06510, U.S.A.
    2. Cell Biology, Yale University School of Medicine, New Haven, CT, 06510, U.S.A.
    Search for more papers by this author

Department of Surgery and Biomedical Engineering Program, BML 365, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520-8062, USA; E-Mail: Bhanu.Jena@Yale.edu

Abstract

Recombinant SNAREs have been demonstrated as the minimal membrane fusion machinery. The participation of additional proteins in the regulation of membrane fusion has been suggested. In this study we provide nanometer-resolution images of native NSF oligomers and SNARE complexes isolated from neurons and the pancreas. Our study reveals the presence of new coiled rod-like structures in association with the SNARE complex only in neuronal tissue. Neuronal SNAREs were found coiled and super-coiled with these structures. The existence of NSF as pentamers in its native state is also demonstrated. The extent of coiling and super-coiling of SNAREs may regulate the potency and efficacy of membrane fusion in cells.

Ancillary