Henk Louagie and Peter Schotte equally contributed to the presented work.
APOPTOSIS INDUCED BY γ IRRADIATION IN PERIPHERAL BLOOD MONONUCLEAR CELLS IS NOT MEDIATED BY CYTOCHROME-C RELEASE AND ONLY PARTIALLY INVOLVES CASPASE-3-LIKE PROTEASES
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 1999 International Federation for Cell Biology
Cell Biology International
Volume 23, Issue 9, pages 611–617, September 1999
How to Cite
Louagie, H., Schotte, P., Vral, A., Cornelissen, M., Thierens, H., Beyaert, R., De Ridder, L. and Philippe, J. (1999), APOPTOSIS INDUCED BY γ IRRADIATION IN PERIPHERAL BLOOD MONONUCLEAR CELLS IS NOT MEDIATED BY CYTOCHROME-C RELEASE AND ONLY PARTIALLY INVOLVES CASPASE-3-LIKE PROTEASES. Cell Biology International, 23: 611–617. doi: 10.1006/cbir.1999.0429
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 9 March 1999; accepted 30 June 1999
- Cited By
- γ irradiation;
- cytochrome c;
- caspase 3;
Caspase 3 has been shown to be actively involved in the apoptotic process in thymocytes after γ-irradiation. We examined caspase 3 activation in mature peripheral blood lymphocytes (PBL) after γ irradiation. Since the activation of caspase 3 is generally prceded by a decrease in mitochondrial membrane potential (ΔΨm) and cytochrome c release, these two parameters were also examined. Apoptosis in PBL after a 5-Gy γ irradiation, is characterized by a decrease in ΔΨm, but surprisingly no release of cytochrome-c and only a weak caspase 3 activation was noticed. In contrast, staurosporin treated PBL showed a decrease in ΔΨm with cytochrome-c release and a clear caspase 3 activation. We were unable to block the decrease in ΔΨm with the caspase-inhibitors zVAD-fmk or zDEVD-fmk after γ irradiation, but DNA fragmentation as measured by the TUNEL assay was partially inhibited. Therefore, in γ irradiated mature PBL, caspase-dependent and -independent pathways, but not cytochrome c, seem to be involved in the apoptotic process.