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Keywords:

  • B16F10 melanoma cells;
  • serum deprivation;
  • PCNA;
  • BrdU;
  • flow cytometry;
  • cell cycle

Abstract

B16F10 murine melanoma cell proliferation was inhibited after 48h in medium with serum in the range 0.1 to 0.5% by volume. Cell viability was mostly retained, whereas cells completely deprived of serum died. Growth-arrested cultures showed serum-dependent suppression of DNA synthesis. The response was typically that of a ‘cell cycle freeze’, verified by flow cytometric distribution of cells. Consequently, serum deprivation did not lead to synchony when serum was restored to arrested populations. Furthermore, there was no change in PCNA expression in arrested cells.